更多"从带有遗传信息的mRNA构建成DNA重组体，要利用"的相关试题:
[多项选择]DNA重组技术中，用来筛选重组体的方法有
A. 标志补救
B. 分子杂交
C. 特异性抗体与产物结合
D. 紫外分光光度计分析
[单项选择]常用于从转化菌中筛选DNA重组体的方法不包括
A. α互补筛选
B. 插入失活筛选
C. 杂交筛选
D. 质粒的小量快速提取
E. DNA序列测定筛选
[单项选择]外源的重组体与感受态细胞混合后，一般作短暂热喇激的温度是
A. 37℃
B. 42℃
C. 57℃
D. 65℃
E. 70℃
[单项选择](1)目的基因的获得 (2)基因的表达(3)载体的制备 (4)基因的转移(5)重组体的制备
A. 1—3—5—4—2
B. 3—4—5—2—1
C. 1—4—3—5—2
D. 3—5—4—2—1
[单项选择]DNA不是
A. DNA复制的模板
B. RNA转录的模板
C. 遗传的物质基础
D. 蛋白质合成的模板
E. 基因的结构基础
[不定项选择]DNA复制需要：①DNA聚合酶Ⅲ，②解链蛋白，③DNA聚合酶Ⅰ，④DNA指导的RNA聚合酶，⑤DNA连接酶，其作用顺序是：（）。
A. ④③①②⑤
B. ②③④①⑤
C. ④②①⑤③
D. ④②①③⑤
E. ②④①③⑤
[单项选择]人工DNA重组中催化外源DNA与载体 DNA连接的酶是
A. 限制性内切酶
B. 限制性外切酶
C. DNA连接酶
D. DNA聚合酶
E. Taq酶
[单项选择]在DNA生物合成中，具有催化RNA指导的DNA聚合反应、RNA水解及DNA指导的 DNA聚合反应三种功能的酶是
A. DNA聚合酶
B. RNA聚合酶
C. 逆转录酶
D. 连接酶
[单项选择]在DNA生物合成中，具有催化RNA指导的DNA聚合反应，RNA水解及DNA指导的DNA聚合反应三种功能的酶是
A. DNA聚合酶
B. RNA聚合酶
C. 逆转录酶
D. 连接酶
[单项选择]进行DNA复制实验时，保留全部DNA复制体系成分，但以DNA聚合酶Ⅱ代替DNA连接酶，试分析可能会出现什么后果
A. DNA高度缠绕，无法作为模板
B. DNA被分解成无数片段
C. 无RNA引物，复制无法进行
D. 随从链的复制无法完成
E. 冈崎片段生成过量
[名词解释]B-form DNA；Z-form DNA
[单项选择]在DNA提取过程中，沉淀DNA用
A. 无水乙醇
B. 乙醚
C. 氯仿
D. 戊醇
[单项选择]抑制DNA回旋酶，使DNA复制受阻，导致DNA降解而细菌死亡的药物是
A. 甲氧苄啶
B. 诺氟沙星
C. 利福平
D. 红霉素
E. 对氨基水杨酸
[单项选择]DNA复制需要：(1)DNA聚合酶Ⅲ；(2)解链蛋白；(3)DNA聚合酶Ⅰ；(4)DNA指导的RNA聚合酶；(5)DNA连接酶参加。其作用的顺序是
A. (4)(3)(1)(2)(5)
B. (4)(2)(1)(3)(5)
C. (2)(3)(4)(1)(5)
D. (2)(4)(1)(3)(5)
E. (2)(4)(3)(1)(5)
[单项选择]第二篇DNA Fingerprinting
DNA is the genetic material found within the cell nuclei of all living things. In mammals the strands of DNA are grouped into structures called chromosomes. With the exception of identical siblings (as in identical twins), the complete DNA of each individual is unique.
DNA fingerprinting is sometimes called DNA typing. It is a method of identification that compares bits of DNA. A DNA fingerprint is constructed by first drawing out a DNA sample from body tissue or fluid such as hair, blood, or saliva. The sample is then segmented using enzymes, and the segments are arranged by size. The segments are marked with probes and exposed on X-ray film, where they form a pattern of black bars — the DNA fingerprint. If the DNA fingerprints produced from two different samples match, the two samples probably came from the same person.
DNA fingerprinting was first developed as an identification technique in 1985. Originally used to detect the presence of genetic diseases, it
A. grouping DNA strands into structures.
B. segmenting DNA with probes
C. constructing body tissues by enzymes.
D. identifying a person by comparing DNAs
