[判断题]核酸探针是指带有标记的一段核酸单链。 ( )

[单项选择]探针是一段带标记的（）
A. 单链DNA
B. 双链DNA
C. cDNA
D. 单链RNA
E. 以上都可以

[多项选择]单链DNA探针的标记可采用( )方法。
A. 用M13噬菌体载体合成
B. 用不对称PCR合成
C. 用缺口平移法合成
D. 用mRNA逆转录合成

[单项选择]利用随机引物法地高辛（DIG）探针标记及检测试剂盒标记探针时，应采取哪种措施。（）
A. 标准标记之前，将模板加热完全变性
B. 标准标记之前，不将模板DNA加热变性
C. 标准标记之前，模板加热可做可不做
D. 标准标记之前，将模板进行碱变性处理
E. 标准标记之前，将模板进行SDS处理

[填空题]为了获得单链标记的DNA探针可以采用以下三种方法：______、______和______。

[单项选择]18米长的竹竿被截成两段，短的一段是长的一段的4/5，那么，短的一段有多少米长( )
A. 7．5米
B. 8米
C. 8．5米
D. 9米

[单项选择]一根长18米的钢筋被锯成两段，短的一段是长的一段的4/5，问短的一段有多少米长？（）
A. 7.5米
B. 8米
C. 8.5米
D. 9米

[单项选择]下面是一段HTML标记，其显示结果是一个______表格。
＜HTML＞
＜HEAD＞
＜TITLE＞基本表格＜/TITLE＞
＜/HEAD＞
＜BODY bgcolor="#FFFFFF"＞
＜TABLE border="1"＞
＜tr＞＜td＞姓名＜/td＞＜td＞学校＜/td＞＜/tr＞
＜tr＞＜td＞张莫＜/td＞＜td＞交大＜/td＞＜/tr＞
＜tr＞＜td＞陈和＜/td＞＜td＞重大＜/td＞＜/tr＞
＜/TABLE＞
＜/BODY＞
＜/HTML＞
A. 三行两列
B. 两行三列
C. 三行三列
D. 两行两列

[单项选择]两根绳子，长的一段是24米，短的一段是18米。两根都截去相同长度的一段后，长的绳子的长度是短绳子长度的4倍。剪短后，长的绳子有多少米( )
A. 6米
B. 2米
C. 8米
D. 4米

[单项选择]下面是一段HTML标记，显示的表的边界宽度是______像素。
＜HTML＞
＜HEAD＞
＜TITLE＞基本表格＜/TITLE＞
＜/HEAD＞
＜BODY bgcolor="#FFFFFF"＞
＜TABLE border="4"width="231"height="131"＞
＜tr＞＜td＞姓名＜/td＞＜td＞学校＜/td＞
＜/tr＞
＜tr＞
＜td＞＜div align="center"＞张莫＜/div＞＜/td＞
＜td＞＜div align="center"＞交大＜/div＞＜/td＞
＜/tr＞
＜tr＞
＜td＞＜div align="right"＞陈和＜/div＞＜/td＞
＜td＞＜div align="right"＞重大＜/div＞＜/td＞
＜/tr＞
＜/TABLE＞
＜/BODY＞
＜/HTML＞
A. 131
B. 231
C. 4
D. 0

[判断题]在Word文档中，删除一个段落标记后，前后两段文字将合并成一段，则段落格式的编排没有变化。 ( )

[单项选择]在Word文档中，删除一个段落标记后，前后两段文字将合并成一段，则段落格式的编排结果（ ）。
A. 没有变化
B. 后一段采用前一段的格式
C. 前一段变成无格式
D. 前一段采用后一段的格式

[单项选择]

听下面一段对话，回答下列问题。
 

听下面一段对话，回答下列问题。

听下面一段对话，回答下列问题。
W: Hi, Bill, can you tell me how it happened
M: Sure. I was mountain climbing in New Hampshire in 1982. Suddenly the weather became really bad. There was a lot of snow and we couldn’t see anything. We got lost. Well, we spent four days on the mountain. The temperature was 20 degrees below zero, We didn’t have any equipment or food.
W: So what happened I guess someone found you, right
M: Yes, but we were very sick. I couldn’t move my legs because of the cold. f spent two months in the hospital. The doctors removed my legs.
W: Right. So you lost your legs, but you want to try your best to stay active.
M: That’s right. In fact, I decided to make some new legs for myself. I realized that no one had to be physically disabled. We can use modern technology to help us.
W: And you built these great new legs. Can you go mountai
A. Design new climbing shoes.
B. Establish a club for the disabled.
C. Use technology to fight his disability.

[单项选择]

听下面一段对话，回答下列问题。
 

听下面一段对话，回答下列问题。
W: Hi, Bill, can you tell me how it happened
M: Sure. I was mountain climbing in New Hampshire in 1982. Suddenly the weather became really bad. There was a lot of snow and we couldn’t see anything. We got lost. Well, we spent four days on the mountain. The temperature was 20 degrees below zero, We didn’t have any equipment or food.
W: So what happened I guess someone found you, right
M: Yes, but we were very sick. I couldn’t move my legs because of the cold. f spent two months in the hospital. The doctors removed my legs.
W: Right. So you lost your legs, but you want to try your best to stay active.
M: That’s right. In fact, I decided to make some new legs for myself. I realized that no one had to be physically disabled. We can use modern technology to help us.
W: And you built these great new legs. Can you go mountain climbing again
M:
A. Low temperature.
B. Terrible weather.
C. An unsuccessful operation.